Journal: Molecular Medicine Reports

Article Title: Electroacupuncture inhibits inflammatory injury by targeting the miR-9-mediated NF-κB signaling pathway following ischemic stroke

doi: 10.3892/mmr.2015.4745

Figure Lengend Snippet: Effect of EA on infarct volume and morphological structure of rat brains. (A) 2,3,5-Triphenyltetrazolium chloride staining indicating cerebral infarct volumes of Sham, MCAO, MCAO + EA, EA + DMSO and EA + miR-9 inhibitors groups. (B) Bar graph showing the percentage of total brain volume in each group (n=4; * P<0.05, vs. the MCAO and the EA + miR-9 inhibitors groups). MCAO, middle cerebral artery occlusion; EA, electroacupuncture; miR-9, microRNA-9; DMSO, dimethyl sulfoxide.

Article Snippet: A SYBR Green ® microRNA Reverse Transcription kit (Guangzhou RiboBio Co., Ltd., Guangzhou, China) was used to reverse transcribe the RNA into cDNA prior to qPCR.

Techniques: Staining

Journal: Molecular Medicine Reports

Article Title: Electroacupuncture inhibits inflammatory injury by targeting the miR-9-mediated NF-κB signaling pathway following ischemic stroke

doi: 10.3892/mmr.2015.4745

Figure Lengend Snippet: HE staining. At the end of the experiment, cerebral tissues from the (A) Sham, (B and C) MCAO, (D) MCAO + EA, (E) EA + DMSO and (F) EA + miR-9 inhibitor groups were processed for HE staining (n=6). The cerebral cortical histopathological changes and degree of inflammatory cell infiltration in the infarct areas of each group were observed under an optical microscope at magnifications of (A and B) ×200 and (C–F) ×400. HE, hematoxylin and eosin; MCAO, middle cerebral artery occlusion; EA, electroacupuncture; DMSO, dimethyl sulfoxide; miR-9, microRNA-9.

Article Snippet: A SYBR Green ® microRNA Reverse Transcription kit (Guangzhou RiboBio Co., Ltd., Guangzhou, China) was used to reverse transcribe the RNA into cDNA prior to qPCR.

Techniques: Staining, Microscopy

Journal: Molecular Medicine Reports

Article Title: Electroacupuncture inhibits inflammatory injury by targeting the miR-9-mediated NF-κB signaling pathway following ischemic stroke

doi: 10.3892/mmr.2015.4745

Figure Lengend Snippet: Effects of EA on the expression of miR-9 in the cerebral cortex. Reverse transcription-quantitative polymerase chain reaction of the miR-9 expression level in the peri-infarct cerebral cortex of the Sham, MCAO, MCAO + EA, EA + DMSO and EA + miR-9 inhibitor groups 72 h following MCAO and reperfusion ( * P<0.05, vs. Sham group; # P<0.05, vs. MCAO and EA + miR-9 inhibitor groups). MCAO, middle cerebral artery occlusion; EA, electroacupuncture; miR-9, microRNA-9; DMSO, dimethyl sulfoxide.

Article Snippet: A SYBR Green ® microRNA Reverse Transcription kit (Guangzhou RiboBio Co., Ltd., Guangzhou, China) was used to reverse transcribe the RNA into cDNA prior to qPCR.

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Molecular Medicine Reports

Article Title: Electroacupuncture inhibits inflammatory injury by targeting the miR-9-mediated NF-κB signaling pathway following ischemic stroke

doi: 10.3892/mmr.2015.4745

Figure Lengend Snippet: Effect of EA on NF-κB signaling pathway-associated factors. (A) Evaluation of NF-κB p65 (green), DAPI (blue) and the colocalization positive cells in peri-infarct cortical tissue of the Sham, MCAO, MCAO + EA, EA + DMSO and EA + miR-9 inhibitor groups (n=4). (B) Western blot analysis of the protein expression levels of NF-κB p65, TNF-α, IL-1β and IκBα in the peri-infarct cortical tissue of the MCAO, MCAO + EA, EA + DMSO and EA + miR-9 inhibitors groups. (C) Bar graph showing the fold change of NF-κB p65, TNF-α, IL-1β and IκBα in each group (n=4) ( * P<0.05, vs. MCAO group; # P<0.05, vs. MCAO + EA and EA + DMSO groups). EA, electroacupuncture; DAPI, 4′,6-diamidino-2-phenylindole; MCAO, middle cerebral artery occlusion; DMSO, dimethyl sulfoxide; miR-9, microRNA-9; NF-κB, nuclear factor-κB; TNF-α, tumor necrosis factor-α; IL-1β, interleukin-1β; IκBα, inhibitor of κBα.

Article Snippet: A SYBR Green ® microRNA Reverse Transcription kit (Guangzhou RiboBio Co., Ltd., Guangzhou, China) was used to reverse transcribe the RNA into cDNA prior to qPCR.

Techniques: Western Blot, Expressing

Journal: Molecular Medicine Reports

Article Title: Electroacupuncture inhibits inflammatory injury by targeting the miR-9-mediated NF-κB signaling pathway following ischemic stroke

doi: 10.3892/mmr.2015.4745

Figure Lengend Snippet: Interaction between miR-9 and NF-κB. (A) The site of miR-9 binding to NF-κB. (B) Luciferase activity of psiCHECK2- NF-κB-luc and psiCHECK2-NF-κB-Mut-luc were detected in primary cortical neuronal cells transfected with miR-9 inhibitors (n=3; * P<0.05, vs. NC inhibitors group). Blank, blank control group; NC inhibitors, cells transfected with negative miR-9 inhibitors group; miR-9 inhibitors, cells transfected with miR-9 inhibitors group. miR-9, microRNA-9; NF-κB, nuclear factor-κB.

Article Snippet: A SYBR Green ® microRNA Reverse Transcription kit (Guangzhou RiboBio Co., Ltd., Guangzhou, China) was used to reverse transcribe the RNA into cDNA prior to qPCR.

Techniques: Binding Assay, Luciferase, Activity Assay, Transfection, Control

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Bostrycin inhibits proliferation of human lung carcinoma A549 cells via downregulation of the PI3K/Akt pathway

doi: 10.1186/1756-9966-30-17

Figure Lengend Snippet: Relative change in expression of microRNA-638 and microRNA-923 in A549 cells treated with bostrycin detected by microRNA real time PCR . A549 cells were treated with 10 μM Bostrycin for 72 h and total RNA was isolated for microRNA real time PCR assay. Expression levels of microRNA-638 and microRNA-923 were determined as described. Untreated A549 cells were used as control. Each condition was repeated 4 times.

Article Snippet: RT-PCR was performed using the TaqMan MicroRNA Reverse Transcription Kit (LC Sciences, USA) and the ABI PRISM 7000 Sequence Detection System (Life Technologies Corporation, Carlsbad, CA, USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Isolation, Control